In vitro and in vivo tests of PLA/d-HAp nanocomposite

Thi Thom Nguyen, Thai Hoang, Van Mao Can, Anh Son Ho, Song Hai Nguyen, Thi Thu Trang Nguyen, Thi Nam Pham, Thu Phuong Nguyen, Thi Le Hien Nguyen and Mai Thanh Dinh Thi

Abstract

The bioactivity of the PLA/d-HAp nanocomposite with 30 wt.% d-HAp was evaluated by in vitro tests and indicated that after 7 immersion days in SBF solution, PLA amorphous part was hydrolyzed and PLA crystal part was remained. The formation of apatite on the surface of the material was observed. The in vivo test results of PLA/d-HAp nanocomposite (70/30 wt/wt) on femur of dogs displayed that 3 months after grafting, the materials did not induce any osteitis, osteomyelitis or structural abnormalities. The histological and x-ray image demonstrated a growth of the bone into the material area, while osteitis and osteomyelitis were not observed