Rapid and sensitive detection of clenbuterol using a fluorescence nanosensor based on diazo coupling mechanism

Thi Thanh Hop Tran, Thi Mai Huong Do, Mai Ha Hoang, Duc Tuyen Nguyen, Quang Tuan Le, Duc Nghia Nguyen and Trinh Tung Ngo

Abstract

In this paper, the fluorescence resonance energy transfer (FRET) effect has been used for fabrication of nanosensor for the detection of clenbuterol. In the nanosensor, the CdTe quantum dots (QDs) are the donors while the acceptor is the super-macromolecule formed by the diazoation coupling mechanism between diazo clenbuterol and naphthylethylene diamine. Changes in fluorescence intensities of nanosensor were used to determine the clenbuterol concentration. We have successfully fabricated a nanosensor for detection of clenbuterol sensible to clenbuterol concentration of 10⁻¹² g ml⁻¹