Biosurfactant assisted silver nanoparticle synthesis: a critical analysis of its drug design aspects

Satya Eswari Jujjavarapu, Swasti Dhagat, Preeti Mishra

Abstract


Silver nanoparticles exhibit wide dimension antimicrobial and antifungal properties apart from their applications in medical and cosmetics sector. They are synthesized by physical, chemical and biological methods. Biological methods are the best technique because of their non-toxic and energy efficient nature. Microbial surfactin are synthesized either on the microbial cell surface or excreted extracellularly and hence are an alternative to synthetic surfactin for the production of silver nanoparticles. Microbial surfactin display anti-tumor and anti-microbial properties, and can also be used as drug delivery agents. In this work, synthesis of surfactin was performed using Bacillus subtilis. The surfactin thus produced was analyzed by emulsification assay, oil spilling test, and hemolytic test. Silver nanoparticles synthesized from surfactant were observed by UV–vis spectroscopy in the range of 400-600 nm. The UV–vis spectra showed a resonance peak at 410 nm corresponding to the peak of silver nanoparticles. The X-ray diffraction pattern showed an intense diffraction peak at 45º with an average particle size of 14 nm. In silico drug designing of surfactin A synthetase C was performed using Schrödinger software for identification of novel drugs. All the two identified ligands, leucine and lysine, exhibited docking with the target protein which shows their potential as drug molecules.

Keywords


Surfactin; silver nanoparticles; green technology; B. subtilis; in silico drug design; surfactin A synthetase C

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Published by: IOP Publishing and Vietnam Academy of Science and Technology
Frequency: Articles appear online as they are published with print copies published 4 times per year
ISSN: Print: 2043-6254; Online: 2043-6262
DOI Range: 10.1088/issn.2043-6262

© Copyright 2016 Advances in Natural Sciences: Nanoscience and Nanotechnology